USP11 Polyklonaler Antikörper

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• KD/KO Validated

USP11 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10244-1-PBS

Synonyme

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  WB analysis of Jurkat using 10244-1-AP (same clone as 10244-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 10244-1-AP (same clone as 10244-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10244-1-AP (same clone as 10244-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10244-1-AP (same clone as 10244-1-PBS)

  IP result of anti-USP11 (IP:10244-1-AP, 4ug; Detection:10244-1-AP 1:300) with HEK-293 cells lysate 1480ug. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10244-1-AP (same clone as 10244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10244-1-AP (USP11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10244-1-PBS bindet in WB, IHC, IP, Indirect ELISA USP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP11 fusion protein Ag0296
• Vollständiger Name: ubiquitin specific peptidase 11
• Berechnetes Molekulargewicht: 963 aa, 110 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC000350
• Gene symbol: USP11
• Gene ID (NCBI): 8237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

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