USP11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP11 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 22340-1-AP

Synonyme

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 22340-1-AP

  WB result of USP11 antibody (22340-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells.

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  WB analysis using 22340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22340-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22340-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22340-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22340-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 22340-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22340-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22340-1-AP

  IP Result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug.

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  IHC staining of human breast cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 22340-1-AP

  Immunofluorescent analysis of HEK-293 cells using 22340-1-AP (USP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, HEK-293-Zellen, humanes Hodengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

22340-1-AP bindet in WB, IP, IHC, IF, ELISA USP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP11 fusion protein Ag17829
• Vollständiger Name: ubiquitin specific peptidase 11
• Berechnetes Molekulargewicht: 963 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC000350
• Gene symbol: USP11
• Gene ID (NCBI): 8237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.

Publikationen

Species	Application	Title
human	WB,IHC	Lab Invest; Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.; Authors - Sheng Zhang ; KD Validated
	WB	Cell Rep; TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.; Authors - Qingyu Luo
human	WB,IF	Cell Death Discov; Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.; Authors - Yang Gao
human	WB,IF	Cell; X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women; Authors - Yan Yan ; KD Validated
human	WB	Sci Adv; Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia; Authors - Qi Jin ; KD Validated
human	IHC,IF	Cell Death Dis; USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1; Authors - Junyi Duan