USP11 Monoklonaler Antikörper

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• KD/KO Validated

USP11 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2G6E7

Kat-Nr. : 67805-1-Ig

Synonyme

Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12

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  WB analysis using 67805-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67805-1-Ig (USP11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67805-1-Ig

  WB result of USP11 antibody (67805-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells.

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  IHC staining of human stomach cancer using 67805-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 67805-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67805-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP11 antibody (67805-1-Ig, Clone: 2G6E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, Daudi-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

67805-1-Ig bindet in WB, IHC, IF/ICC, ELISA USP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP11 fusion protein Ag19058
• Vollständiger Name: ubiquitin specific peptidase 11
• Berechnetes Molekulargewicht: 963 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC000350
• Gene symbol: USP11
• Gene ID (NCBI): 8237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

Publikationen

Species	Application	Title
human	IF	Angiogenesis; Endothelial USP11 drives VEGFR2 signaling and angiogenesis via PRDX2/c-MYC axis; Authors - Can Chen