USP13 Monoklonaler Antikörper

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USP13 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6E8C10

Kat-Nr. : 66176-1-PBS

Synonyme

6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3

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  WB analysis of HeLa using 66176-1-Ig (same clone as 66176-1-PBS)

  WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  WB analysis using 66176-1-Ig (same clone as 66176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66176-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA USP13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP13 fusion protein Ag10506
• Vollständiger Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Berechnetes Molekulargewicht: 863 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97-100 kDa
• GenBank-Zugangsnummer: BC016146
• Gene symbol: USP13
• Gene ID (NCBI): 8975
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.