USP14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP14 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 14517-1-AP

Synonyme

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

---

Galerie der Validierungsdaten

• 

  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14517-1-AP

  WB result of USP14 antibody (14517-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

• 

  WB analysis of HeLa using 14517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14517-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14517-1-AP

  IP Result of anti-USP14 (IP:14517-1-AP, 4ug; Detection:14517-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IF Staining of HepG2 using 14517-1-AP

  Immunofluorescent analysis of HepG2 cells, using USP14 antibody 14517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 14517-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug USP14 antibody (14517-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, Mausherzgewebe, Neuro-2a-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:18000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

14517-1-AP bindet in WB, IP, IHC, IF, FC, ELISA USP14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP14 fusion protein Ag5979
• Vollständiger Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 52-60 kDa
• GenBank-Zugangsnummer: BC003556
• Gene symbol: USP14
• Gene ID (NCBI): 9097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).

Publikationen

Species	Application	Title
human	WB,IF	Autophagy; USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination.; Authors - Arishya Sharma
pig	WB,IF,IP	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming ; KD Validated
human	IF	Cell Prolif; SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.; Authors - Jun Xu
human	WB	Front Pharmacol; SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis.; Authors - Tingting Zhu
human	IHC	J Biol Chem; The USP14-NLRC5 pathway inhibits titanium particle-induced osteolysis in mice by suppressing NF-κB and PI3K/AKT activities.; Authors - Guibin Fang
human	WB,IHC	Cancer Med; PMID: 30296012; Authors - Ying Fu ; KD Validated