USP14 Monoklonaler Antikörper

USP14 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B12G3

Kat-Nr. : 67746-1-PBS

Synonyme

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

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  WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67746-1-Ig (same clone as 67746-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP14 antibody (67746-1-Ig, Clone: 3B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67746-1-PBS bindet in WB, IF/ICC, Indirect ELISA USP14 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP14 fusion protein Ag6312
• Vollständiger Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC003556
• Gene symbol: USP14
• Gene ID (NCBI): 9097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.