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USP14 Rekombinanter Antikörper

USP14 Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G12

Kat-Nr. : 82128-1-PBS

Synonyme

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

Filter:
  • Western Blot (WB) analysis of various lysates using USP14 Recombinant antibody (82128-1-RR)

    WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP14 Recombinant antibody (82128-1-RR)

    WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using USP14 Recombinant antibody (82128-1-RR)

    IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82128-1-PBS

    Affinity and Kinetic Characterization of 82128-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

82128-1-PBS bindet in WB, IP, Indirect ELISA USP14 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen USP14 fusion protein Ag5979
Vollständiger Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerBC003556
Gene symbol USP14
Gene ID (NCBI) 9097
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.