USP14 Rekombinanter Antikörper

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• KD/KO Validated

USP14 Rekombinant Antikörper für WB, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

3G12

Kat-Nr. : CL750-82128

Synonyme

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

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  WB analysis using CL750-82128

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using CL750-82128

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using CL750-82128

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated USP14 Recombinant antibody (CL750-82128, Clone:3G12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-82128 bindet in WB, FC (Intra) USP14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: USP14 fusion protein Ag5979
• Vollständiger Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC003556
• Gene symbol: USP14
• Gene ID (NCBI): 9097
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.