USP15 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

USP15 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1C9G3

Rezensionen (1)

Kat-Nr. : 67557-1-Ig

Synonyme

1C9G3, Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15

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  WB analysis using 67557-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67557-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of HeLa using 67557-1-Ig

  WB result of USP15 antibody (67557-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP15 transfected HeLa cells.

• 

  WB analysis of 4T1 using 67557-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67557-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67557-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 67557-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP15 antibody (67557-1-Ig, Clone: 1C9G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

67557-1-Ig bindet in WB, IHC, IF, IP, CoIP, ELISA USP15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP15 fusion protein Ag5712
• Vollständiger Name: ubiquitin specific peptidase 15
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 112 kDa
• GenBank-Zugangsnummer: BC020688
• Gene symbol: USP15
• Gene ID (NCBI): 9958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IF	Cell Death Dis; The deubiquitinating enzyme USP15 stabilizes ERα and promotes breast cancer progression.; Authors - Xiaohong Xia ; KD Validated
human	WB	Endocr Relat Cancer; LncRNA MFSD4A-AS1 promotes lymphangiogenesis and lymphatic metastasis of papillary thyroid cancer; Authors - Xiaoli Liu
human	WB,CoIP	J Cancer; USP15 promotes the progression of papillary thyroid cancer by regulating HMGB1 stability through its deubiquitination; Authors - Si-Si Wang ; KD Validated
mouse,human	WB,IP,IHC,IF	Nat Commun; Proteostatic reactivation of the developmental transcription factor TBX3 drives BRAF/MAPK-mediated tumorigenesis; Authors - Zhenlei Zhang
human	WB	ACS Pharmacol Transl Sci; Cell-Based Covalent-Capture Deubiquitinase Assay for Inhibitor Discovery; Authors - Megan N Doleschal
mouse,human	WB,IHC,IF	Mol Med; USP15 regulates radiation-induced DNA damage and intestinal injury through K48-linked deubiquitination and stabilisation of ATM; Authors - Ruiqiu Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shunbin (Verified Customer) (06-07-2023)

Great antibody for Western Blot. Very specific single band

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000

USP15 Antibody Western Blot validation (1:5,000 dilution) in (Cat no:67557-1-Ig)