USP16 Polyklonaler Antikörper

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• KD/KO Validated

USP16 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14055-1-AP

Synonyme

Deubiquitinating enzyme 16, Ubiquitin thiolesterase 16, UBP M, USP16

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Galerie der Validierungsdaten

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  WB analysis using 14055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14055-1-AP (USP16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14055-1-AP

  IP Result of anti-USP16 (IP:14055-1-AP, 5ug; Detection:14055-1-AP 1:1000) with HeLa cells lysate 880ug.

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  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14055-1-AP (USP16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14055-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14055-1-AP (USP16 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14055-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA USP16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP16 fusion protein Ag4981
• Vollständiger Name: ubiquitin specific peptidase 16
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC030777
• Gene symbol: USP16
• Gene ID (NCBI): 10600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP16 (Ubiquitin specific peptidase 16) was originally identified as a DUB of histone H2A which is implicated in cell cycle progression and HOXD10 gene expression (PMID:17914355). The knockout study has reported that depletion of USP16 results in early embryonic lethality in mice (PMID:24784029). Moreover, USP16 mediated the deubiquitination and stabilization of Drp1 through its direct interaction with Drp1 (PMID:37488647).

Publikationen

Species	Application	Title
human	WB,IF	J Cell Sci; A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase; Authors - Nadine Sen Nkwe
mouse	WB,CoIP	Arthritis Res Ther; Deubiquitinase USP16 induces gouty arthritis via Drp1-dependent mitochondrial fission and NLRP3 inflammasome activation; Authors - Qingdong Wang ; KD Validated
human	WB,IF	J Cell Sci; A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase.; Authors - Nadine Sen Nkwe ; KD Validated
human	IHC	Rheumatology (Oxford); Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.; Authors - Christine Galant
human	WB,IP	Nat Commun; FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice; Authors - Gaozan Tong ; KD Validated