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USP18 Polyklonaler Antikörper

USP18 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12153-1-PBS

Synonyme

43 kDa ISG15-specific protease, EC:3.4.19.12, hUBP43, ISG15-specific-processing protease, ISG43

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using USP18 Polyclonal antibody (12153-1-AP)

    WB analysis of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

    WB result of USP18 antibody(12153-1-AP) with MCF-7 cells (Control and CRISPR USP18). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using USP18 Polyclonal antibody (12153-1-AP)

    IHC staining of human colon cancer using 12153-1-AP (same clone as 12153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12153-1-AP (USP18 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using USP18 Polyclonal antibody (12153-1-AP)

    IF Staining of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP18 antibody (12153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12153-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA USP18 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen USP18 fusion protein Ag2785
Vollständiger Name ubiquitin specific peptidase 18
Berechnetes Molekulargewicht 372 aa, 43 kDa
Beobachtetes Molekulargewicht 43 kDa, 70 kDa
GenBank-ZugangsnummerBC014896
Gene symbol USP18
Gene ID (NCBI) 11274
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).