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  • KD/KO Validated

USP19 Polyklonaler Antikörper

USP19 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 25768-1-AP

Synonyme

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
Erfolgreiche IPJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25768-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA USP19 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen USP19 fusion protein Ag22646
Vollständiger Name ubiquitin specific peptidase 19
Berechnetes Molekulargewicht 1318 aa, 146 kDa
Beobachtetes Molekulargewicht 70 kDa, 120 kDa, 150-180 kDa
GenBank-ZugangsnummerBC146752
Gene symbol USP19
Gene ID (NCBI) 10869
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for USP19 antibody 25768-1-APProtokoll herunterladen
IP protocol for USP19 antibody 25768-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
IHC

Clin Transl Med

Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer

Authors - Xiaoqiang Zhang
humanWB,IP,IHC

Mol Ther

Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin

Authors - Apoorvi Tyagi
  • KD Validated
humanWB

J Cell Biol

USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites.

Authors - Peiyuan Chai
humanWB

Theranostics

USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2

Authors - Arun Pandian Chandrasekaran
  • KD Validated
human

Cell Mol Life Sci

Enhanced secretion of the amyotrophic lateral sclerosis ALS-associated misfolded TDP-43 mediated by the ER-ubiquitin specific peptidase USP19

Authors - Flavien Picard