USP19 Polyklonaler Antikörper

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USP19 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25768-1-PBS

Synonyme

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19

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  WB analysis of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 25768-1-AP (same clone as 25768-1-PBS)

  WB result of USP19 antibody (25768-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP19 transfected HeLa cells. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

  IP result of anti-USP19 (IP:25768-1-AP, 4ug; Detection:25768-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25768-1-PBS bindet in WB, IP, Indirect ELISA USP19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP19 fusion protein Ag22646
• Vollständiger Name: ubiquitin specific peptidase 19
• Berechnetes Molekulargewicht: 1318 aa, 146 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 120 kDa, 150-180 kDa
• GenBank-Zugangsnummer: BC146752
• Gene symbol: USP19
• Gene ID (NCBI): 10869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).