USP25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP25 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12199-1-PBS

Synonyme

---

• 

  WB analysis using 12199-1-AP (same clone as 12199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 12199-1-AP (same clone as 12199-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

• 

  IP experiment of RAW 264.7 using 12199-1-AP (same clone as 12199-1-PBS)

  IP result of anti-USP25 (IP:12199-1-AP, 4ug; Detection:12199-1-AP 1:300) with RAW 264.7 cells lysate 1600ug. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12199-1-PBS bindet in WB, IHC, IP, Indirect ELISA USP25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP25 fusion protein Ag2837
• Vollständiger Name: ubiquitin specific peptidase 25
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC015930
• Gene symbol: USP25
• Gene ID (NCBI): 29761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.