USP25 Monoklonaler Antikörper

USP25 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6A2

Kat-Nr. : 67769-1-Ig

Synonyme

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

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Galerie der Validierungsdaten

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  WB analysis using 67769-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67769-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 67769-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67769-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, 4T1-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67769-1-Ig bindet in WB, IHC, ELISA USP25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP25 fusion protein Ag28067
• Vollständiger Name: ubiquitin specific peptidase 25
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC015930
• Gene symbol: USP25
• Gene ID (NCBI): 29761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.