USP25 Monoklonaler Antikörper

USP25 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6A2

Kat-Nr. : 67769-1-PBS

Synonyme

Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, Ubiquitin carboxyl-terminal hydrolase 25, EC:3.4.19.12, Deubiquitinating enzyme 25

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  WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67769-1-PBS bindet in WB, IHC, Indirect ELISA USP25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP25 fusion protein Ag28067
• Vollständiger Name: ubiquitin specific peptidase 25
• Berechnetes Molekulargewicht: 125 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC015930
• Gene symbol: USP25
• Gene ID (NCBI): 29761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.