USP30 Polyklonaler Antikörper

USP30 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15402-1-AP

Synonyme

Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12

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  WB analysis using 15402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15402-1-AP (USP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 15402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of hela using 15402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of HepG2 using 15402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HepG2-Zellen, hTERT-RPE1-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A549-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15402-1-AP bindet in WB, IHC, IF/ICC, ELISA USP30 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP30 fusion protein Ag7620
• Vollständiger Name: ubiquitin specific peptidase 30
• Berechnetes Molekulargewicht: 58.5 kDa
• Beobachtetes Molekulargewicht: 59-67 kDa
• GenBank-Zugangsnummer: BC004868
• Gene symbol: USP30
• Gene ID (NCBI): 84749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).

Publikationen

Species	Application	Title
human,mouse	WB,IF	Biochem Biophys Res Commun; USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury; Authors - Yang Wu
	IF	Nature; Immune evasion through mitochondrial transfer in the tumour microenvironment; Authors - Hideki Ikeda
human	IHC	Biochim Biophys Acta Mol Basis Dis; FOXF1 promotes ovarian cancer metastasis by facilitating HMGA2-mediated USP30-dependent S100A6 deubiquitination; Authors - Xi Xu
human	WB	J Biochem Mol Toxicol; USP30 Aggravating the Malignant Progression of Breast Cancer and Its Resistance to Tamoxifen by Inhibiting the Ubiquitination of TOMM40; Authors - Xinran Gao