USP33 Rekombinanter Antikörper

USP33 Rekombinant Antikörper für IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242535A6

Kat-Nr. : 84958-1-PBS

Synonyme

242535A6, EC:3.4.19.12, hVDU1, KIAA1097, Ubiquitin carboxyl-terminal hydrolase 33

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  Sandwich ELISA standard curve of MP01703-3

  Sandwich ELISA standard curve of MP01703-3, Human USP33 Recombinant Matched Antibody Pair - PBS only. 84958-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14268. 84958-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01703-3

  The mean USP33 concentration was determined to be 8.27 ng/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load, 4.70 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 3.49 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load.

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  IF Staining of HeLa using 84958-1-RR (same clone as 84958-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP33 antibody (84958-1-RR, Clone: 242535A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84958-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84958-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84958-1-RR against Human USP33 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84958-1-PBS bindet in IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test USP33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: USP33 fusion protein Ag14268
• Vollständiger Name: ubiquitin specific peptidase 33
• Berechnetes Molekulargewicht: 942 aa, 107 kDa
• GenBank-Zugangsnummer: BC016663
• Gene symbol: USP33
• Gene ID (NCBI): 23032
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.