USP35 Polyklonaler Antikörper

USP35 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 24559-1-AP

Synonyme

EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34

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  WB analysis using 24559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24559-1-AP (USP35 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

24559-1-AP bindet in WB, IHC, CoIP, ELISA USP35 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP35 fusion protein Ag20002
• Vollständiger Name: ubiquitin specific peptidase 35
• Berechnetes Molekulargewicht: 1018 aa, 113 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC131489
• Gene symbol: USP35
• Gene ID (NCBI): 57558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).

Publikationen

Species	Application	Title
human	WB	Open Life Sci; The deubiquitinating enzyme USP35 regulates the stability of NRF2 protein; Authors - Dian Zhang
human	WB	Commun Biol; Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1; Authors - Shuo Wang ; KD Validated
human	WB,CoIP	Am J Physiol Cell Physiol; USP35 promotes breast cancer progression by regulating PFK-1 ubiquitination to mediate glycolysis; Authors - Weibin Lian
human	WB	Transl Oncol; USP35 promotes hepatocellular carcinoma proliferation through GASC1-mediated ROCK2 upregulation; Authors - Gen Chen ; KD Validated
human	WB,IHC,CoIP	Cell Death Discov; Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis; Authors - Guowen Lin
human		Oncogenesis; USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer; Authors - Yi Xiao