USP35 Polyklonaler Antikörper

USP35 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24559-1-PBS

Synonyme

EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34

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  WB analysis using 24559-1-AP (same clone as 24559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24559-1-AP (USP35 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24559-1-PBS bindet in WB, IHC, Indirect ELISA USP35 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP35 fusion protein Ag20002
• Vollständiger Name: ubiquitin specific peptidase 35
• Berechnetes Molekulargewicht: 1018 aa, 113 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC131489
• Gene symbol: USP35
• Gene ID (NCBI): 57558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).