USP36 Rekombinanter Antikörper

USP36 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241962H7

Kat-Nr. : 84620-3-PBS

Synonyme

241962H7, Deubiquitinating enzyme 36, DUB1, EC:2.3.2.-, KIAA1453

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  WB analysis using 84620-3-RR (same clone as 84620-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84620-3-RR (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84620-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01428-1

  Sandwich ELISA standard curve of MP01428-1, Human USP36 Recombinant Matched Antibody Pair - PBS only. 84620-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6411. 84620-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01428-1

  The mean USP36 concentration was determined to be 4,390.7 pg/mL in HeLa cell extract based on a 1.3 mg/mL extract load, 16,332.6 pg/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 3,553.0 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP01428-1

  Cytometric bead array standard curve of MP01428-1, USP36 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84620-3-PBS. Detection antibody: 84620-1-PBS. Standard: Ag6411. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84620-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84620-3-RR against Human USP36 were performed. The affinity constant is 3.71 nM.

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Geprüfte Anwendungen

Produktinformation

84620-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test USP36 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: USP36 fusion protein Ag6411
• Vollständiger Name: ubiquitin specific peptidase 36
• Berechnetes Molekulargewicht: 1123 aa, 123 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC038983
• Gene symbol: USP36
• Gene ID (NCBI): 57602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.