USP39 Polyklonaler Antikörper

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• KD/KO Validated

USP39 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 23865-1-AP

Synonyme

65K, CGI 21, HSPC332, PRO2855, SAD1, SAD1 homolog, SNRNP65, USP39

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 23865-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23865-1-AP

  WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells.

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  WB analysis using 23865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23865-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23865-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 23865-1-AP

  IP Result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IHC staining of human colon cancer using 23865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 23865-1-AP

  Immunofluorescent analysis of HUVEC cells using 23865-1-AP (USP39 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• RIP: See 3 publications below

Produktinformation

23865-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA USP39 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP39 fusion protein Ag20894
• Vollständiger Name: ubiquitin specific peptidase 39
• Berechnetes Molekulargewicht: 565 aa, 65 kDa
• Beobachtetes Molekulargewicht: 60~65 kDa
• GenBank-Zugangsnummer: BC067273
• Gene symbol: USP39
• Gene ID (NCBI): 10713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	RIP,IHC	Mol Oncol; Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation.; Authors - Yue Xiao ; KD Validated
human	WB,IF	Am J Cancer Res; USP39 facilitates breast cancer cell proliferation through stabilization of FOXM1; Authors - Zhenwang Zhang ; KD Validated
human	WB,IHC	Oncotarget; Overexpression of USP39 predicts poor prognosis and promotes tumorigenesis of prostate cancer via promoting EGFR mRNA maturation and transcription elongation.; Authors - Yi Huang ; KD Validated
human	WB,IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
human	WB	Cancer Biomark; Knockdown of USP39 by lentivirus-mediated RNA interference suppresses the growth of oral squamous cell carcinoma; Authors - Ke-Yi Li
human	RIP	Genes (Basel); The Deubiquitinase USP39 Promotes Esophageal Squamous Cell Carcinoma Malignancy as a Splicing Factor.; Authors - Xiaolin Zhu