USP4 Monoklonaler Antikörper

USP4 Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1G11G8

Kat-Nr. : 66822-1-PBS

Synonyme

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

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  WB analysis using 66822-1-Ig (same clone as 66822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  WB analysis using 66822-1-Ig (same clone as 66822-1-PBS)

  HEK-293 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66822-1-Ig (same clone as 66822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66822-1-Ig (same clone as 66822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66822-1-Ig (same clone as 66822-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 66822-1-Ig (same clone as 66822-1-PBS)

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human USP4 (66822-1-Ig, Clone:1G11G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66822-1-PBS bindet in WB, IHC, IF-P, FC (Intra), ELISA USP4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP4 fusion protein Ag28297
• Vollständiger Name: ubiquitin specific peptidase 4 (proto-oncogene)
• Berechnetes Molekulargewicht: 963 aa, 109 kDa
• Beobachtetes Molekulargewicht: 109 kDa
• GenBank-Zugangsnummer: BC125131
• Gene symbol: USP4
• Gene ID (NCBI): 7375
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP4, also named as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a Deubiquitinating enzymes that links to mitogen-activated protein kinase signaling, pre-mRNA splicing and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).