USP46 Polyklonaler Antikörper

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• KD/KO Validated

USP46 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 13502-1-AP

Synonyme

Deubiquitinating enzyme 46, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 46, Ubiquitin thioesterase 46, Ubiquitin thiolesterase 46

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  WB analysis of human kidney using 13502-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13502-1-AP (USP46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 13502-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13502-1-AP (USP46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

13502-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA USP46 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP46 fusion protein Ag4330
• Vollständiger Name: ubiquitin specific peptidase 46
• Berechnetes Molekulargewicht: 366 aa, 42 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC037574
• Gene symbol: USP46
• Gene ID (NCBI): 64854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	WB,IP	Nat Commun; The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling; Authors - Victoria H Ng
mouse	WB	J Exp Med; USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses.; Authors - Zhongxia Yu ; KD Validated
human	WB,IP,IF,CoIP	Biochim Biophys Acta Mol Basis Dis; USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis; Authors - Shun Gao
mouse	WB,IF	Mol Neurobiol; Ceftriaxone Modulates Ubiquitination of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole Propionic Acid Receptors to Improve Long-Term Potentiation Impairment Induced by Exogenous β-Amyloid in a Glutamate Transporter-1 Dependent Manner; Authors - Rui Jiang
human	WB,IHC,IF,CoIP	Exp Cell Res; Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma.; Authors - Maoqing Tian ; KD Validated
human	WB	FEBS Open Bio; Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins; Authors - Kazuma Yoshioka