USP48 Polyklonaler Antikörper
USP48 Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 12076-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | PC-3-Zellen, HeLa-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Pankreaskarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Produktinformation
12076-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA USP48 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | USP48 fusion protein Ag2749 |
Vollständiger Name | ubiquitin specific peptidase 48 |
Berechnetes Molekulargewicht | 1035 aa, 119 kDa |
Beobachtetes Molekulargewicht | 119 kDa |
GenBank-Zugangsnummer | BC013567 |
Gene symbol | USP48 |
Gene ID (NCBI) | 84196 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for USP48 antibody 12076-1-AP | Protokoll herunterladen |
IHC protocol for USP48 antibody 12076-1-AP | Protokoll herunterladenl |
IP protocol for USP48 antibody 12076-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis | ||
Cancer Res YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis. | ||
Rheumatology (Oxford) Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β. | ||
Eur J Pain USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice | ||
FASEB J Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination | ||
Sci Rep RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31 |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rachel (Verified Customer) (09-10-2020) | Antibody detected band of the expected height by western blot.
|