USP48 Polyklonaler Antikörper

USP48 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12076-1-AP

Synonyme

Deubiquitinating enzyme 48, EC:3.4.19.12, RAP1GA1, Ubiquitin carboxyl-terminal hydrolase 48, Ubiquitin thiolesterase 48



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, HeLa-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Pankreaskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12076-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA USP48 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen USP48 fusion protein Ag2749
Vollständiger Name ubiquitin specific peptidase 48
Berechnetes Molekulargewicht 1035 aa, 119 kDa
Beobachtetes Molekulargewicht 119 kDa
GenBank-ZugangsnummerBC013567
Gene symbol USP48
Gene ID (NCBI) 84196
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for USP48 antibody 12076-1-APProtokoll herunterladen
IHC protocol for USP48 antibody 12076-1-APProtokoll herunterladenl
IP protocol for USP48 antibody 12076-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IP,IHC,IF

Acta Pharm Sin B

Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis

Authors - Can Cheng
WB

Cancer Res

YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.

Authors - Shuai Ye
humanIHC

Rheumatology (Oxford)

Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.

Authors - Christine Galant
mouseIP

Eur J Pain

USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice

Authors - Jiacheng Yu
mouseWB

FASEB J

Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination

Authors - Ping Wang
humanCoIP

Sci Rep

RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31

Authors - Wei Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Rachel (Verified Customer) (09-10-2020)

Antibody detected band of the expected height by western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T