USP49 Polyklonaler Antikörper

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• KD/KO Validated

USP49 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 18066-1-AP

Synonyme

Ubiquitin-specific-processing protease 49, Ubiquitin thiolesterase 49, Ubiquitin thioesterase 49, Ubiquitin carboxyl-terminal hydrolase 49, EC:3.4.19.12

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  WB analysis of MCF-7 using 18066-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18066-1-AP (USP49 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18066-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18066-1-AP (USP49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 18066-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18066-1-AP (USP49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18066-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18066-1-AP (USP49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 16 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

18066-1-AP bindet in WB, IHC, IP, CoIP, ChIP, ELISA USP49 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP49 fusion protein Ag12697
• Vollständiger Name: ubiquitin specific peptidase 49
• Berechnetes Molekulargewicht: 73 kDa, 79 kDa
• Beobachtetes Molekulargewicht: 79-85 kDa
• GenBank-Zugangsnummer: BC014176
• Gene symbol: USP49
• Gene ID (NCBI): 25862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USPs are cysteine proteases that vary greatly in size and structural complexity. Outside of the core catalytic domain, USPs exhibit N-terminal and C-terminal extensions that have been proposed to play roles in determining cellular localization and substrate specificity of these enzymes(PMID:14715245). USP49(Ubiquitin carboxyl-terminal hydrolase 49) belongs to this family. It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis.; Authors - Kamini Kaushal ; KO Validated
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
human	WB	Theranostics; USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2; Authors - Arun Pandian Chandrasekaran ; KD Validated
human	WB	Elife; USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication.; Authors - Ting Pan ; KD Validated
human	WB,CoIP	Carcinogenesis; USP49 promotes adenocarcinoma of the esophagogastric junction malignant progression via activating SHCBP1-β-catenin-GPX4 axis; Authors - Yun Ding ; KD Validated
human	WB	J Cell Mol Med; CCAT1/FABP5 promotes tumour progression through mediating fatty acid metabolism and stabilizing PI3K/AKT/mTOR signalling in lung adenocarcinoma.; Authors - Jing Chen