USP5 Polyklonaler Antikörper

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• KD/KO Validated

USP5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 10473-1-AP

Synonyme

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

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Galerie der Validierungsdaten

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  WB analysis of A549 using 10473-1-AP

  WB result of USP5 antibody (10473-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.

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  WB analysis using 10473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 10473-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 10473-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10473-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10473-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10473-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10473-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 10473-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10473-1-AP

  IP Result of anti-USP5 (IP:10473-1-AP, 4ug; Detection:10473-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, DU 145-Zellen, humanes Herzgewebe, humanes Kolongewebe, Maushirngewebe, U2OS-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 4 publications below
• ELISA: See 2 publications below

Produktinformation

10473-1-AP bindet in WB, IP, IHC, IF, ELISA USP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP5 fusion protein Ag0770
• Vollständiger Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC004889
• Gene symbol: USP5
• Gene ID (NCBI): 8078
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 has 2 isoforms with the molecular mass of 96 kDa and 93 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity; Authors - Hiroaki Ohishi
		Blood; The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.; Authors - Zubin Zhang
human	WB	Theranostics; Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor.; Authors - Xin Xu ; KD Validated
human	WB	Clin Transl Med; MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.; Authors - Mengqing Xiao ; KD Validated
human	WB	Acta Pharmacol Sin; Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.; Authors - Xue-Han Chen
rat	WB,IP	Sci Signal; Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.; Authors - Sonja L Joksimovic