USP5 Polyklonaler Antikörper

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USP5 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15158-1-AP

Synonyme

Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT, ISOT 1

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  WB analysis of mouse lung using 15158-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15158-1-AP

  WB result of USP5 antibody (15158-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.

• 

  WB analysis of MDA-MB-453s using 15158-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15158-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 15158-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15158-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15158-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15158-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15158-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15158-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15158-1-AP (USP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15158-1-AP

  IP result of anti-USP5 (IP:15158-1-AP, 4ug; Detection:15158-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, A549-Zellen, HeLa-Zellen, humanes Hirngewebe, MDA-MB-453s-Zellen, Rattenhirngewebe, Rattenlungengewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15158-1-AP bindet in WB, IF, IP, ELISA USP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP5 fusion protein Ag7252
• Vollständiger Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC005139
• Gene symbol: USP5
• Gene ID (NCBI): 8078
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Publikationen

Species	Application	Title
mouse	WB,IP,IF	Neuron; The Deubiquitinating Enzyme USP5 Modulates Neuropathic and Inflammatory Pain by Enhancing Cav3.2 Channel Activity.; Authors - Agustin García-Caballero
human	WB	J Exp Med; A pathological missense mutation in the deubiquitinase USP5 leads to insensitivity to pain; Authors - Flavia T T Antunes
	WB	FASEB J; PPP2R1B is modulated by ubiquitination and is essential for spermatogenesis.; Authors - Mufeng Du
mouse	WB	Toxicology; Critical role of Cav3.2 T-type calcium channels in the peripheral neuropathy induced by bortezomib, a proteasome-inhibiting chemotherapeutic agent, in mice.; Authors - Shiori Tomita