USP5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

USP5 Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F12C10

Kat-Nr. : 66213-1-PBS

Synonyme

3F12C10, Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT

---

• 

  WB analysis using 66213-1-Ig (same clone as 66213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66213-1-Ig (same clone as 66213-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

  WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66213-1-Ig (same clone as 66213-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP51440-1

  Cytometric bead array sample test of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS.

• 

  Cytometric bead array standard curve of MP51440-1

  Cytometric bead array standard curve of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS. Standard:Ag7575. Range: 1.563-200 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

66213-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA USP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP5 fusion protein Ag7575
• Vollständiger Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC005139
• Gene symbol: USP5
• Gene ID (NCBI): 8078
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.