USP50 Polyklonaler Antikörper

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USP50 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20374-1-PBS

Synonyme

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  WB analysis of mouse brain using 20374-1-AP (same clone as 20374-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20374-1-AP (same clone as 20374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20374-1-AP (same clone as 20374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20374-1-AP (same clone as 20374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 20374-1-AP (same clone as 20374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 20374-1-AP (same clone as 20374-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20374-1-AP (USP50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20374-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA USP50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin specific peptidase 50
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: NM_203494
• Gene symbol: USP50
• Gene ID (NCBI): 373509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function through targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.