USP50 Polyklonaler Antikörper

USP50 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30239-1-AP

Synonyme

Ubiquitin-specific peptidase 50, Ubiquitin carboxyl-terminal hydrolase 50, EC:3.4.19.12

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  WB analysis using 30239-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30239-1-AP (USP50 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30239-1-AP bindet in WB, ELISA USP50 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP50 fusion protein Ag31275
• Vollständiger Name: ubiquitin specific peptidase 50
• Berechnetes Molekulargewicht: 339 aa, 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC146493
• Gene symbol: USP50
• Gene ID (NCBI): 373509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP50 (Ubiquitin Specific Peptidase 50) is a deubiquitinating enzyme that belongs to the USP family. It plays a key role in DNA replication, genome stability and cell cycle regulation. Recent studies have found that USP50 maintains the stability of the DNA replication process and telomere integrity by regulating the use of nuclease and deconjugating enzymes. During replication, USP50 was able to inhibit the harmful DNA2 nuclease activity and substitute the use of RecQ deconjugase, thereby preventing replication fork stalling and DNA damage. In addition, USP50 plays a role in the cell cycle G2/M phase transition, assembly of the NLRP3 inflammasome complex, and organization of nuclear speckles. Its loss of function leads to DNA double-strand breaks and telomere instability in cells under replication stress. These findings provide new insights into the understanding of the pathogenesis of genetic diseases and may offer potential targets for the treatment of diseases such as cancer and premature aging.