USP7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 26948-1-AP

Synonyme

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

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  WB analysis using 26948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 26948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 26948-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26948-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26948-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26948-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26948-1-AP (USP7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 26948-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26948-1-AP (USP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26948-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26948-1-AP (USP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, K-562-Zellen, Mausmilzgewebe, NIH/3T3-Zellen, PC-12-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• RIP: See 1 publications below

Produktinformation

26948-1-AP bindet in WB, IHC, RIP, ELISA USP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP7 fusion protein Ag25634
• Vollständiger Name: ubiquitin specific peptidase 7 (herpes virus-associated)
• Beobachtetes Molekulargewicht: 126-128 kDa
• Gene symbol: USP7
• Gene ID (NCBI): 7874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	Cancer Res; Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.; Authors - Monika Vishnoi
human	WB	Front Immunol; Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.; Authors - Wenying Gao ; KD Validated
human	WB,IHC	Int J Biol Sci; Flap endonuclease 1 Facilitated Hepatocellular Carcinoma Progression by Enhancing USP7/MDM2-mediated P53 Inactivation.; Authors - Saiyan Bian
human	WB	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
mouse	WB,IHC	Front Pharmacol; Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice; Authors - Yu-Hui Gu
human	WB	ACS Pharmacol Transl Sci; Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf; Authors - Hongyue Li