USP7 Monoklonaler Antikörper

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USP7 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D7D11

Kat-Nr. : 66514-1-PBS

Synonyme

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

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  WB analysis using 66514-1-Ig (same clone as 66514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66514-1-Ig (same clone as 66514-1-PBS)

  WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected HeLa cells. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66514-1-Ig (same clone as 66514-1-PBS)

  WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected A549 cells. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66514-1-Ig (same clone as 66514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66514-1-Ig (same clone as 66514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66514-1-Ig (same clone as 66514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66514-1-Ig (same clone as 66514-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66514-1-Ig (USP7 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66514-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66514-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA USP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP7 fusion protein Ag25652
• Vollständiger Name: ubiquitin specific peptidase 7 (herpes virus-associated)
• Beobachtetes Molekulargewicht: 128 kDa
• Gene symbol: USP7
• Gene ID (NCBI): 7874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.