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USP8 Rekombinanter Antikörper

USP8 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240016D3

Kat-Nr. : 83172-2-PBS

Synonyme

240016D3, EC:3.4.19.12, hUBPy, HumORF8, KIAA0055

Filter:
  • Western Blot (WB) analysis of various lysates using USP8 Recombinant antibody (83172-2-RR)

    WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00188-1

    Sandwich ELISA standard curve of MP00188-1

    Sandwich ELISA standard curve of MP00188-1, Human USP8 Recombinant Matched Antibody Pair - PBS only. 83172-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27143. 83172-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00188-1

    Sample test of MP00188-1

    The mean USP8 concentration was determined to be 41.15 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 47.12 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using USP8 Recombinant antibody (83172-2-RR)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using USP8 Recombinant antibody (83172-2-RR)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP8 Recombinant antibody (83172-2-RR)

    IF Staining of HeLa using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using USP8 Recombinant antibody (83172-2-RR)

    FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-1-PBS. Standard: Ag27143. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83172-2-RR

    Affinity and Kinetic Characterization of 83172-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

83172-2-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test USP8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen USP8 fusion protein Ag27143
Vollständiger Name ubiquitin specific peptidase 8
Berechnetes Molekulargewicht 1118 aa, 128 kDa
Beobachtetes Molekulargewicht128 kDa
GenBank-ZugangsnummerBC110590
Gene symbol USP8
Gene ID (NCBI) 9101
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP8 is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage.