USP9X Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP9X Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(45)

Rezensionen (1)

Kat-Nr. : 55054-1-AP

Synonyme

Deubiquitinating enzyme FAF-X, DFFRX, EC:3.4.19.12, FAF, FAM

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  WB analysis using 55054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55054-1-AP (USP9X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55054-1-AP (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 55054-1-AP

  IP result of anti-USP9X (IP:55054-1-AP, 4ug; Detection:55054-1-AP 1:1000) with HeLa cells lysate 1280 ug.

• 

  IP experiment of HeLa using 55054-1-AP

  IP result of anti-USP9X (IP:55054-1-AP, 4ug; Detection:55054-1-AP 1:4000) with HeLa cells lysate 2000 ug.

• 

  IHC staining of human pancreas cancer using 55054-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55054-1-AP (USP9X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 55054-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55054-1-AP (USP9X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55054-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP9X antibody (55054-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 55054-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug USP9X Polyclonal antibody (55054-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 38 publications below
• IHC: See 5 publications below
• IF: See 9 publications below
• IP: See 6 publications below
• CoIP: See 6 publications below

Produktinformation

55054-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA USP9X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin specific peptidase 9, X-linked
• Berechnetes Molekulargewicht: 292 kDa
• Beobachtetes Molekulargewicht: 260-290 kDa
• GenBank-Zugangsnummer: NM_001039590
• Gene symbol: USP9X
• Gene ID (NCBI): 8239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1; Authors - Wenfeng Ren
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.; Authors - Athea Vichas ; KO Validated
human	IF	Nat Commun; Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.; Authors - Michael Dietachmayr
human	WB,IP,IF	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li ; KO Validated
human	WB	Cell Death Differ; USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.; Authors - Xiaohua Jie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Echo (Verified Customer) (03-20-2024)

The antibody quality was pretty good. Band was clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: HEK293T

USP9X Antibody Western Blot validation (1:10000 dilution) in HEK293T (Cat no:55054-1-AP)