USP9X Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

USP9X Rekombinant Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B23

Kat-Nr. : 81892-1-PBS

Synonyme

1B23, Deubiquitinating enzyme FAF-X, DFFRX, EC:3.4.19.12, FAF

---

• 

  WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 81892-1-RR (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

  WB result of USP9X antibody (81892-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP9X transfected HEK-293 cells. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 81892-1-RR (same clone as 81892-1-PBS)

  IP result of anti-USP9X (IP:81892-1-RR, 4ug; Detection:81892-1-RR 1:3000) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 81892-1-RR (same clone as 81892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81892-1-RR (same clone as 81892-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP9X antibody (81892-1-RR, Clone: 1B23 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81892-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

81892-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA USP9X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ubiquitin specific peptidase 9, X-linked
• Berechnetes Molekulargewicht: 292 kDa
• Beobachtetes Molekulargewicht: 260-290 kDa
• GenBank-Zugangsnummer: NM_001039590
• Gene symbol: USP9X
• Gene ID (NCBI): 8239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.