UTP18 Rekombinanter Antikörper

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• KD/KO Validated

UTP18 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241240F1

Kat-Nr. : 84035-3-PBS

Synonyme

241240F1, CGI 48, WDR50

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  WB analysis of HeLa using 84035-3-RR (same clone as 84035-3-PBS)

  WB result of UTP18 antibody (84035-3-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UTP18 transfected HeLa cells. This data was developed using the same antibody clone with 84035-3-PBS in a different storage buffer formulation.

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  Sample test of MP00941-2

  The mean UTP18 concentration was determined to be 22.3 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  IF Staining of A431 using 84035-3-RR (same clone as 84035-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using UTP18 antibody (84035-3-RR, Clone: 241240F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84035-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84035-3-RR (same clone as 84035-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using UTP18 antibody (84035-3-RR, Clone: 241240F1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84035-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00941-2

  Cytometric bead array standard curve of MP00941-2, UTP18 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84035-3-PBS. Detection antibody: 84035-4-PBS. Standard: Ag34662. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84035-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84035-3-RR against Human UTP18 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84035-3-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test UTP18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UTP18 fusion protein Ag34662
• Vollständiger Name: UTP18, small subunit (SSU) processome component, homolog (yeast)
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: NM_016001
• Gene symbol: UTP18
• Gene ID (NCBI): 51096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UTP18 (UTP18 small subunit processome component), also known as WDR50. It is expected to be located in nucleus. The protein is one part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. The calculated molecular weight of UTP18 is 62 kDa.