UTP18 Rekombinanter Antikörper

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• KD/KO Validated

UTP18 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241240F1

Kat-Nr. : 84035-3-RR

Synonyme

241240F1, CGI 48, WDR50

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  WB analysis of HeLa using 84035-3-RR

  WB result of UTP18 antibody (84035-3-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UTP18 transfected HeLa cells.

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  IF Staining of A431 using 84035-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using UTP18 antibody (84035-3-RR, Clone: 241240F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84035-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using UTP18 antibody (84035-3-RR, Clone: 241240F1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 84035-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84035-3-RR against Human UTP18 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84035-3-RR bindet in WB, IF/ICC, ELISA UTP18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UTP18 fusion protein Ag34662
• Vollständiger Name: UTP18, small subunit (SSU) processome component, homolog (yeast)
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: NM_016001
• Gene symbol: UTP18
• Gene ID (NCBI): 51096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UTP18 (UTP18 small subunit processome component), also known as WDR50. It is expected to be located in nucleus. The protein is one part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. The calculated molecular weight of UTP18 is 62 kDa.