UTP3 Polyklonaler Antikörper

UTP3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18886-1-AP

Synonyme

Charged amino acid-rich leucine zipper 1, CRL1, CRLZ1, SAS10

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  WB analysis of A549 using 18886-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18886-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18886-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18886-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18886-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 18886-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using UTP3 antibody (18886-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Hirngewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18886-1-AP bindet in WB, IF/ICC, ELISA UTP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UTP3 fusion protein Ag13454
• Vollständiger Name: UTP3, small subunit (SSU) processome component, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 479 aa, 55 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC004546
• Gene symbol: UTP3
• Gene ID (NCBI): 57050
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.