UTY Rekombinanter Antikörper

UTY Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241626F7

Kat-Nr. : 84332-5-RR

Synonyme

UTY1, KDM6C, KDM6AL, Histone demethylase UTY, 241626F7

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  WB analysis using 84332-5-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84332-5-RR (UTY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDA-MB-231 using 84332-5-RR

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using UTY antibody (84332-5-RR, Clone: 241626F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84332-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84332-5-RR against Human UTY were performed. The affinity constant is 0.23 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84332-5-RR bindet in WB, IF/ICC, ELISA UTY und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UTY fusion protein Ag35806
• Vollständiger Name: ubiquitously transcribed tetratricopeptide repeat gene, Y-linked
• Berechnetes Molekulargewicht: 150kDa，1347aa
• Beobachtetes Molekulargewicht: 130-160kDa
• GenBank-Zugangsnummer: NM_007125.4
• Gene symbol: UTY
• Gene ID (NCBI): 7404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UTY (Ubiquitously Transcribed Tetratricopeptide Repeat Containing, Y-Linked) is a protein-coding gene located on the Y chromosome. It is the Y-linked homolog of the X-linked gene KDM6A, which encodes a histone demethylase. UTY is involved in the regulation of gene expression and chromatin structure, similar to its X-linked counterpart KDM6A (PMID: 31097364). UTY is expressed in various tissues and is predicted to be involved in transcription, translation, and nucleic acid binding, which are central to the regulation of gene expression during development, immune function, cell proliferation, and differentiation.