UXT Polyklonaler Antikörper

UXT Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11047-1-AP

Synonyme

ART 27, Protein UXT, UXT

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11047-1-AP (UXT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11047-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11047-1-AP (UXT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11047-1-AP

  IP Result of anti-UXT (IP:11047-1-AP, 3ug; Detection:11047-1-AP 1:300) with mouse brain tissue lysate 4000ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11047-1-AP bindet in WB, IP, ELISA UXT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UXT fusion protein Ag1513
• Vollständiger Name: ubiquitously-expressed transcript
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 18 kDa, 45 kDa
• GenBank-Zugangsnummer: BC008890
• Gene symbol: UXT
• Gene ID (NCBI): 8409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitously expressed transcript (UXT) is a gene transcription regulator. It can regulate androgen receptor(AR) transcription by concerting with the coreperssor UR1. It is proposed a potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling, for UXT increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC. UXT is possible a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function. It can suppresses cell transformation and mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM

Publikationen

Species	Application	Title
mouse	WB	Nature; Long non-coding antisense RNA controls Uchl1 translation through an embedded SINEB2 repeat.; Authors - Carrieri Claudia C