Ubiquilin 1 Rekombinanter Antikörper

Ubiquilin 1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242092E9

Kat-Nr. : 84649-2-PBS

Synonyme

UBQLN1, 242092E9, DA41, DSK2, hPLIC 1

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  WB analysis using 84649-2-RR (same clone as 84649-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84649-2-RR (Ubiquilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84649-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84649-2-RR (same clone as 84649-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ubiquilin 1 antibody (84649-2-RR, Clone: 242092E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84649-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01462-2

  Cytometric bead array standard curve of MP01462-2, Ubiquilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84649-2-PBS. Detection antibody: 84649-3-PBS. Standard: Ag17530. Range: 0.469-30 ng/mL.

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  Cytometric bead array standard curve of MP01462-1

  Cytometric bead array standard curve of MP01462-1, Ubiquilin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84649-2-PBS. Detection antibody: 84649-1-PBS. Standard:Ag17530. Range: 0.469-30 ng/mL.

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  Affinity and Kinetic Characterization of 84649-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84649-2-PBS against Human Ubiquilin 1 was performed. The affinity constant is 0.99 nM.

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  Affinity and Kinetic Characterization of 84649-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84649-2-RR against Human Ubiquilin 1 were performed. The affinity constant is 1.08 nM.

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Geprüfte Anwendungen

Produktinformation

84649-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA Ubiquilin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ubiquilin 1 fusion protein Ag17530
• Vollständiger Name: ubiquilin 1
• Berechnetes Molekulargewicht: 589 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-71 kDa
• GenBank-Zugangsnummer: BC010066
• Gene symbol: Ubiquilin 1
• Gene ID (NCBI): 29979
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.