Ubiquilin 1 Rekombinanter Antikörper

Ubiquilin 1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242092E9

Kat-Nr. : 84649-2-RR

Synonyme

UBQLN1, hPLIC-1, hPLIC1, hPLIC 1, DSK2

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  WB analysis using 84649-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84649-2-RR (Ubiquilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84649-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ubiquilin 1 antibody (84649-2-RR, Clone: 242092E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84649-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84649-2-RR against Human Ubiquilin 1 were performed. The affinity constant is 1.08 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, L02-Zellen, MOLT-4-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84649-2-RR bindet in WB, IF/ICC, ELISA Ubiquilin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ubiquilin 1 fusion protein Ag17530
• Vollständiger Name: ubiquilin 1
• Berechnetes Molekulargewicht: 589 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-71 kDa
• GenBank-Zugangsnummer: BC010066
• Gene symbol: Ubiquilin 1
• Gene ID (NCBI): 29979
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.