Uromodulin Rekombinanter Antikörper

Uromodulin Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242258H4

Kat-Nr. : 85597-4-PBS

Synonyme

Tamm-Horsfall urinary glycoprotein, THP, Umod, Uromodulin, secreted form

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  WB analysis using 85597-4-RR (same clone as 85597-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85597-4-RR (Umod antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85597-4-RR (Uromodulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Uromodulin antibody (85597-4-RR, Clone: 242258H4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Uromodulin antibody (85597-4-RR, Clone: 242258H4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85597-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA Uromodulin und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Uromodulin fusion protein Eg2748
• Vollständiger Name: Uromodulin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_009470.5
• Gene symbol: Umod
• Gene ID (NCBI): 22242
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin is linked to water/electrolyte balance and kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)