Uteroglobin/CC10 Polyklonaler Antikörper

Uteroglobin/CC10 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26909-1-PBS

Synonyme

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  WB analysis of rat lung using 26909-1-AP (same clone as 26909-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 26909-1-AP (same clone as 26909-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 26909-1-AP (same clone as 26909-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26909-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Uteroglobin/CC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Uteroglobin/CC10 fusion protein Ag25530
• Vollständiger Name: secretoglobin, family 1A, member 1 (uteroglobin)
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 9 kDa
• GenBank-Zugangsnummer: BC004481
• Gene symbol: Uteroglobin
• Gene ID (NCBI): 7356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.