V5-tag Polyklonaler Antikörper

V5-tag Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (2)

Anwendung

WB, RIP, IP, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Rezensionen (5)

Kat-Nr. : 14440-1-AP

Synonyme

GKPIPNPLLGLDST, V5, V5 tag, V5-tag

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Recombinant protein using 14440-1-AP

  IP Result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche IP: Rekombinantes Protein Protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 26 publications below
• IF: See 2 publications below
• IP: See 5 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14440-1-AP bindet in WB, RIP, IP, IF, ChIP, ELISA V5-tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: V5-tag
• Berechnetes Molekulargewicht: 1 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Publikationen

Species	Application	Title
	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
	WB	Sci Adv; The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals; Authors - Liuke Sun
	IF	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
	WB	Nucleic Acids Res; HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.; Authors - Xiaochuan Liu
human	IP,WB	Nucleic Acids Res; CGG repeats trigger translational frameshifts that generate aggregation-prone chimeric proteins.; Authors - Shannon E Wright
	WB	Cell Death Differ; Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.; Authors - Kunlun Yin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

The V5 antibodys shows clear band in western blots. But if you use this antibody for IP, the background is so strong that it is hard for you to find your band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Nick (Verified Customer) (04-20-2022)

Product for IP was used and had to use above the recommended concentrations

• Applications: Western Blot, Immunoprecipitation

FH

Ben (Verified Customer) (10-12-2021)

Worked well, we got good images from it even at a very low dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: iAstrocyte

FH

Daniela (Verified Customer) (01-08-2020)

In immunoblot of HeLa total cell lysate, this antibody gives several no specific bands in the negative control sample (cell lysate from no transfected cells). Whereas it doesn't recognize any specific protein in cell lysate from HeLa transfected with a plasmid for exogenous protein in frame with V5-tag. In immunofluorescence of transfected HeLa cells this antibody give a very faint signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000 for immunoblot and 1:200 for immunofluorescence
• Cell Tissue Type: HeLa cells

FH

Erica (Verified Customer) (12-11-2019)

I used this antibody for westtern blot of V5 tagged protein and it worked very well. The signal is very strong and there isn't background. Highly recommend

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000 WB
• Cell Tissue Type: MEF