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VAMP1 Polyklonaler Antikörper

VAMP1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13115-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of human brain tissue using VAMP1 Polyclonal antibody (13115-1-AP)

    WB analysis of human brain using 13115-1-AP (same clone as 13115-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13115-1-AP (VAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAMP1 Polyclonal antibody (13115-1-AP)

    IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAMP1 Polyclonal antibody (13115-1-AP)

    IHC staining of mouse brain using 13115-1-AP (same clone as 13115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using VAMP1 Polyclonal antibody (13115-1-AP)

    IF Staining of SH-SY5Y using 13115-1-AP (same clone as 13115-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 13115-1-AP (VAMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13115-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

13115-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VAMP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen VAMP1 fusion protein Ag3787
Vollständiger Name vesicle-associated membrane protein 1 (synaptobrevin 1)
Berechnetes Molekulargewicht 117 aa, 13 kDa
Beobachtetes Molekulargewicht 13 kDa
GenBank-ZugangsnummerBC023286
Gene symbol VAMP1
Gene ID (NCBI) 6843
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP1, also named as synaptobrevin 1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP1 is a part of the SNARE (soluble NSF-attachment protein receptor) complex. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP1 is involved in synaptic vesicle exocytosis, a fundamental step in neurotransmitter release.