VAMP2 Polyklonaler Antikörper

VAMP2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, Blocking, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 10135-1-AP

Synonyme

SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Galerie der Validierungsdaten

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  WB analysis using 10135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10135-1-AP (VAMP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 10135-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10135-1-AP (VAMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10135-1-AP (VAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10135-1-AP (VAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10135-1-AP (VAMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10135-1-AP (VAMP2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 11 publications below

Produktinformation

10135-1-AP bindet in WB, IHC, IF, Blocking, ELISA VAMP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAMP2 fusion protein Ag0179
• Vollständiger Name: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC002737
• Gene symbol: VAMP2
• Gene ID (NCBI): 6844
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Publikationen

Species	Application	Title
mouse	WB	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB,IF	Adv Sci (Weinh); Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage; Authors - Nikita Raj
	IF	Neuron; Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.; Authors - Roberta Cagnetta
mouse	IHC,IF	Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan
human	IF	Sci Signal; A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity; Authors - Hyunbae Kim