VAMP2 Polyklonaler Antikörper

VAMP2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Ziege / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 30001-0-AP

Synonyme

SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Galerie der Validierungsdaten

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ELISA: See 3 publications below

Produktinformation

30001-0-AP bindet in WB, ELISA VAMP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Ziege / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: NM_014232
• Gene symbol: VAMP2
• Gene ID (NCBI): 6844
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family of proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2 consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Publikationen

Species	Application	Title
human	ELISA	J Cell Biochem; The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells.; Authors - Shi Xuerong X
human	ELISA	J Cell Biochem; TCEP treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells.; Authors - Shi Xuerong X
human	ELISA	J Cell Biochem; Un-nicked BoNT/B activity in human SHSY-5Y neuronal cells.; Authors - Shi Xuerong X
human	WB	Mol Neurobiol; L116 Deletion in CSPα Promotes α-Synuclein Aggregation and Neurodegeneration; Authors - Tao Guo