VAMP2 Monoklonaler Antikörper
VAMP2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG3
Getestete Reaktivität
Hausschwein, human, Kaninchen, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1G7E8
Kat-Nr. : 67822-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Kaninchenhirngewebe, Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe |
| Erfolgreiche Detektion in IHC | Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | U-87 MG-Zellen, SH-SY5Y-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
67822-1-Ig bindet in WB, IHC, IF/ICC, ELISA VAMP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Kaninchen, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG3 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | VAMP2 fusion protein Ag17908 |
| Vollständiger Name | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| Berechnetes Molekulargewicht | 13 kDa |
| Beobachtetes Molekulargewicht | 19 kDa |
| GenBank-Zugangsnummer | BC002737 |
| Gene symbol | VAMP2 |
| Gene ID (NCBI) | 6844 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for VAMP2 antibody 67822-1-Ig | Protokoll herunterladen |
| IHC protocol for VAMP2 antibody 67822-1-Ig | Protokoll herunterladenl |
| IF protocol for VAMP2 antibody 67822-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Michela (Verified Customer) (06-20-2024) | The immunofluorescence was carried out in primary hippocampal neurons with an antibody dilution of 1:500. The antibody appears to be very specific and with minimal background staining.
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